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Determination of qRT-PCR primer amplification specificity for (A) ACTB, (B) eIF4A, (C) GAPDH, (D) HPRT1, (E) IPO8, (F) PPIA, (G) RPLP1, (H) RPL13A, (I) TBP and (J) YWHAZ based on melt curve analysis. Single melt curve indicated that only the desired product was specifically amplified in each reaction. d(RFU)/dT = Relative fluorescence units (RFU) with time (T).
