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. 2019 Jul 5;18(3):1745–1751. doi: 10.3892/etm.2019.7736

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Copyright: © Che et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of taraxasterol on apoptosis rate and protein expression of BAX, cleaved caspase-3 and p53. HT-29 cells were treated with 10 µg/ml LPS, then treated with 2.5, 5 or 10 mg/ml taraxasterol. (A) Cells were analyzed by flow cytometry and (B) the apoptosis rate (%) was calculated. (C) Representative images from western blot analysis of apoptosis-related proteins and quantification of protein expression levels of (D) BAX, (E) cleaved caspase-3 and (F) p53, as a ratio to β-actin. ^#P<0.05 vs. control; *P<0.05 vs. LPS only group. LPS, lipopolysaccharide; BAX, bcl-2-associated X protein; p53, tumor protein p53.