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. 2019 Jul 3;18(3):1831–1836. doi: 10.3892/etm.2019.7729

Figure 5.

Figure 5.

SCAL1 overexpression inhibited iNOS expression and reduced NO production in lung cells under high-glucose conditions. (A) The relative SCAL1 expression level was determined by reverse transcription-quantitative PCR in the BEAS-2B normal human lung cell line following transfection with SCAL1 expression vector and treatment with high glucose. (B) The relative iNOS protein expression level was determined by western blotting in BEAS-2B cells following transfection with SCAL1 expression vector and treatment with high glucose. (C) NO production was examined in the supernatant of BEAS-2B cells following transfection with SCAL1 expression vector and treatment with high glucose. *P<0.05 as indicated by lines. SCAL1, smoke and cancer-associated lncRNA 1; iNOS, inducible nitric oxide synthase; NO, nitric oxide; C, control; NC, negative control.