Figure 2.

Vasorin (VASN) expression promoted cell growth in glioma cells. A, Expression levels of VASN in glioma cell lines. B, The ectopic expression of the VASN gene was confirmed by quantitative real‐time PCR (qPCR). C, The ectopic expression and protein localization of the VASN was confirmed by western blot. The knockdown efficiency of VASN shRNA was confirmed by qPCR (D) and western blot (E). CCK‐8 assays were performed at different time points to detect the effect of knockdown (F) ectopic expression (G) of VASN on the growth of glioma cells. (H, I) Colony‐forming assays were performed on the same cell lines with either VASN knockdown or ectopic expression. The histograms illustrate the number of colonies counted. Data are presented as mean ± SD of 3 independent experiments. *P < 0.05, **P < 0.01. PSIN, negative control of ectopic expression of VASN; shSC, negative control of shRNA of VASN