Table 1.
Expression of miR‐31 in human colorectal tumor tissues were evaluated by performing TaqMan real‐time PCR assays
| Colorectal tumor | n | Overexpression of miR‐31 | P‐value |
|---|---|---|---|
| Low‐grade adenoma | 28 | 6 (21.4%) | 0.008 |
| High‐grade adenoma | 18 | 3 (16.6%) | |
| Cancer (M) | 10 | 1 (10.0%) | |
| Cancer (SM) | 12 | 7 (58.3%) | |
| Advanced cancer | 44 | 25 (56.8%) | |
| Adenoma and cancer (M) | 56 | 10 (17.9%) | 0.001 |
| Cancer (SM and advanced) | 56 | 32 (57.1%) |
The relative expression levels were calculated using the ΔΔCt method, with RNU6B used as a control. The relative expression level in normal tissue was indicated as “1.” The expression levels in tumors were designated as upregulated when the fold change from the expression in the non‐tumorous tissue was 10.0. Statistical differences in miRNA levels were evaluated by using Pearson's χ2 test and, Fisher's exact test for differences between 2 groups. A P‐value of 0.05 was considered to be significant; statistical analysis was done in this manner for all subsequent tables. M, carcinoma in situ; SM, carcinoma invades submucosa.