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. 2019 Jul 3;110(8):2471–2484. doi: 10.1111/cas.14094

Figure 6.

Figure 6

A, Flow cytometric analysis showing that XBP1 silencing effectively increased the radiation‐induced cell apoptotic index in oropharyngeal squamous cell carcinoma (OSCC) (FaDu and Detroit562) cells. B, Western blot results showing that transfection of XBP1 siRNA effectively increased cleaved caspase‐3 and cleaved poly(ADP‐ribose) polymerase (PARP) expression in OSCC (FaDu and Detroit562) cells. C, Flow cytometric analysis showing that 4μ8c effectively increased the radiation‐induced cell apoptotic index in OSCC (FaDu and Detroit562) cells and that interleukin‐6 (IL‐6) reversed this effect. D, Western blot results showing that 4μ8c effectively increased cleaved caspase‐3 and cleaved PARP expression in OSCC (FaDu and Detroit562) cells and that IL‐6 reversed this effect. E, Western blot results showing that tocilizumab effectively increased cleaved caspase‐3 and cleaved PARP expression in OSCC (FaDu and Detroit562) cells. F, CCK‐8 cell proliferation assay results showing that transfection of XBP1 siRNA and treatment with 4μ8c effectively reduced cell proliferation and that IL‐6 reversed this effect. The band densities were quantified with ImageJ software and normalized to those of the loading control, β‐actin