Skip to main content
. 2019 Jul 25;110(8):2629–2642. doi: 10.1111/cas.14104

Figure 4.

Figure 4

5‐Aza‐dC treatment inhibits DNA methylation of Tmem54 DMR and upregulates mRNA expression in Hepa1c1c7 cells and GRX cells. Expression of Tmem54 was measured in Hepa1c1c7 cells treated with different doses of 5‐aza‐dC (A) or GRX cells treated with 50 μmol/L of 5‐aza‐dC (C) by real‐time PCR. The methylation level of each CpG site in the DMR was validated in Hepa1c1c7 cells (B) and GRX cells (D) by bisulfite sequencing