Figure 2.

Genetic PIN1 suppression affected pancreatic ductal carcinoma (PDAC) cell functions in vitro. Stable PIN1 knockdown (KD) of PANC1 and BXPC3 cells with RNA interference and their controls were used, respectively. A, PIN1 KD cells resulted in marked reduction of PIN1 expression determined by immunoblot. B, PIN1 KD decreased in cell proliferation. C, D, PIN1 KD decreased cell colony formation. E, F, PIN1 KD reduced both migration and invasion. G, Cell lysates of PIN1 KD and controls were performed by immunoblot with specific antibodies. (All experiments were performed independently at least 3 times. Data are shown as mean ± SEM. One‐way ANOVA and Student's t test were used; *P < 0.05, **P < 0.01.)