Figure 3.

High expression of microRNA (miR)‐30c is related to the increased percentage of M1 cells within tumor‐associated macrophages (TAMs). A, Percentage of M1 cells (defined as CD68⁺ and inducible nitric oxide synthase [iNOS]⁺) with gastric cancer (GC) TAMs were determined by using flow cytometry. The comparison was undertaken between miR‐30c^Low and miR‐30c^High GC TAMs. B, Percentage of M1 cells with GC TAMs was determined by using immunofluorescence staining (×100), the integrated optical density value of each figure was obtained by using Image J analyzing 5 random visions. All experiments were undertaken in triplicate. Data are presented as mean ± SD. Student's t test. MFI, mean fluorescence intensity