Figure 5.

ATP stimulates hypoxia‐inducible factor (HIF)‐2α signaling via AKT pathway. A, Western blotting illustrated that HIF1A‐siRNA did not affect phosphoglycerate kinase 1 (PGK1) or HIF‐2α expression. B, Western blotting demonstrated that ATP‐induced elevation of HIF‐2α was abolished by AKT‐siRNA. C, Western blotting demonstrated that ATP‐induced expression changes of HIF‐2α and target proteins were attenuated by LY294002 (AKT inhibitor). D, Western blotting illustrated that phosphorylation of AKT could be regulated by HIF2A‐siRNA (left) or Flag‐HIF‐2α (right). E, Western blotting proved that P2Y2‐siRNA attenuated ATP‐mediated alterations in HIF‐2α signaling