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. 2019 Jun 26;123(2):e226–e238. doi: 10.1016/j.bja.2019.05.027

Fig. 2.

Fig. 2

Effect of mitochondrial calcium uniporter inhibition on mitochondrial calcium in spinal cord dorsal horn (SCDH) neurones. Ruthenium 360 (Ru360) (50 μM) was injected intrathecally every morning 30 min before morphine injection once a day for 7 days. At 30 min after the last morphine, Rhod-2/AM was intrathecally injected for Rhod-2 imaging. (a–d) Representative image of Rhod-2 in the SCDH in (a) saline+sham, (b) Ru360+sham, (c) saline+morphine anti-nociceptive tolerance (MT), and (d) Ru360+MT groups. (e) There was a significant increase in Rhod-2-positive cells at SCDH Laminae I and II in the vehicle+MT group compared with the vehicle+sham or Ru360+sham groups (**P<0.01; one-way anova; n=6); Rhod-2-positive cells in the Ru360+MT group was lower than that in the vehicle+MT group in SCDH Laminae I and II (P<0.05; one-way anova; n=6). Similarly, there was a significant increase in Rhod-2-positive cells in the vehicle+MT group compared with the vehicle+sham or Ru360+sham group in (f) Laminae III–V or (g) Laminae I–V (***P<0.001; one-way anova; n=6). (f) Rhod-2-positive cells in Ru360+MT group were fewer than in the vehicle+MT group in SCDH Laminae III–V (**P<0.01; one-way anova; n=6). (g) Total Rhod-2-positive cells in Laminae I–V in the Ru360+MT group was lower than in the vehicle+MT group (**P<0.01; one-way anova; n=6).