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. 2019 Jul 1;18(3):1645–1652. doi: 10.3892/etm.2019.7717

Figure 3.

Figure 3.

Downregulation of miR-125a-5p increases the expression levels of NLRP3, ASC, caspase-1, IL-1β and CCL4 in HVSMCs. The cells were trasfected with miR-125a-5p inhibitor and NC inhibitor, and treated with ox-LDL. (A) The miR-125a-5p levels were detected by RT-qPCR. (B) RT-qPCR and (C) western blot assays were performed to analyse NLRP3, ASC, caspase-1, IL-1β and CCL4 mRNA and protein expression levels. ***P<0.001 vs. ox-LDL + HVSMCs. HVSMC, human vascular smooth muscle cells; miR, microRNA; IL, interleukin; ox-LDL, oxidised low-density lipoprotein; NLRP3, NACHT, LRR and PYD domains-containing protein 3; α-SMA, smooth muscle actin; RT-q, reverse transcription-quantitative; CCL4, C-C motif chemokine 4-like; NC, negative control; ASC, apoptosis associated speck-like protein.