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. 2019 Jul 1;18(3):1645–1652. doi: 10.3892/etm.2019.7717

Figure 5.

Figure 5.

Knockdown of CCL4 attenuated the induction of NLRP3, ASC, caspase-1 and IL-1β expression by the miR-125a-5p inhibitor. human vascular smooth muscle cells were co-transfected with si-CCL4 and miR-125a-5p inhibitor for 48 h. (A) Western blot and (B) reverse transcription-quantitative PCR assays were performed to analyse CCL4 mRNA and protein expression levels. (C) The NLRP3, ASC, caspase-1 and IL-1β expression levels were detected by western blot assays. *P<0.05, **P<0.01 and ***P<0.001 vs. miR-125a-5p inhibitor. CCL4, C-C motif chemokine 4-like; NC, negative control; IL, interleukin; miR, microRNA; si, small interfering; NLRP3, NACHT, LRR and PYD domains-containing protein 3; ASC, apoptosis associated speck-like protein.