Figure 3.

Effects of SB216763 on the phosphorylation of β-catenin in MC3T3-E1 cells. The cultured cells were treated with various doses of SB216763 for 120 min. The cell extracts were subjected to SDS-PAGE with subsequent western blotting with antibodies against phospho-specific β-catenin, β-catenin or GAPDH. The histogram shows the quantitative representations of the levels of phosphorylated-β-catenin after normalization with respect to GAPDH obtained from a laser densitometric analysis. The levels were expressed as the fold increase to the basal levels presented as lane 1. The representative result of triplicate independent experiments are presented.