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. 2019 Jul 10;18(3):1921–1927. doi: 10.3892/etm.2019.7764

Figure 4.

Figure 4.

Effects of Wnt3a and SB on the T3-induced expression of Bglap2 mRNA in MC3T3-E1 cells. The cultured cells were pretreated with 30 ng/ml of Wnt3a, 10 µM of SB or vehicle for 60 min and subsequently stimulated by 10 nM of T3 or vehicle for 48 h. The respective total RNA was subsequently isolated and transcribed into cDNA. The expression of Bglap2mRNA and GAPDH mRNA were quantified by reverse transcription-quantitative PCR. The Bglap2 mRNA levels were normalized to those of GAPDH mRNA. Each value represents the mean ± SEM of triplicate determinations from three independent cell preparations. *P<0.05 vs. the value of the control; #P<0.05 vs. the value of T3 alone. SB, SB216763; T3, triiodothyronine.