Figure 5.

Effects of Wnt3a and SB on the T₃-stimulated transactivation activity of the thyroid hormone response element in MC3T3-E1 cells. The cultured cells were pretreated with 30 ng/ml of Wnt3a, 10 µM of SB or vehicle for 60 min at 6 h after the transfection with the pDR4-Luc reporter plasmid (1 µg/dish). The cells were then stimulated by 10 nM of T₃ or vehicle for 48 h. The measurement of the luciferase activity of the cell lysates were performed using a dual luciferase reporter assay system. Each value represents the mean ± SEM of triplicate determinations from three independent cell preparations. *P<0.05 vs. the value of the control; ^#P<0.05 vs. the value of T₃ alone. SB, SB216763; T₃, triiodothyronine.