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. 2019 Jul 10;18(3):1701–1714. doi: 10.3892/etm.2019.7763

Figure 3.

Figure 3.

Determination of the effect of treatment with SB203580 in H9c2 cells in high-glucose and sI/R condition. The H9c2 cell, which induced high-glucose condition, was treated with or without 10 µM p38 MAPK inhibitor (SB203580) for 24 h. (A) Cell viability was measured by an MTT cell survival assay. The H9c2 cell induced high-glucose was treated with SB203580 for 24 h. (B) Then, the cell was simulated ischemia for 40 min followed by 24 h of reperfusion. *P<0.05 vs. NG+sI/R; #P<0.05 vs. HG+sI/R as determined by ANOVA. (C) The H9c2 cells were induced with high-glucose condition and treatment with SB203580 supplemented with 2′7-dichlorofluorescein diacetate dye for 24 h. Intracellular ROS production was measured. Each bar graph represents the mean ± SEM. *P<0.05 vs. NG; #P<0.05 vs. HG as determined by ANOVA. ROS, reactive oxygen species; NG, normal glucose; HG, high glucose; sI/R, simulated ischemia/reperfusion.