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. 2019 Jul 1;18(3):1863–1869. doi: 10.3892/etm.2019.7720

Figure 5.

Figure 5.

H19 may function through the Bax, Bcl2, caspase 3 and the PI3K/AKT pathway in FTC-133 cells. Thyroid cancer cells were transfected with H19 siRNA, NC or control, respectively. Reverse transcription-quantitative polymerase chain reaction analysis of (A) Bax, (B) Bcl-2 and (C) caspase 3 expression was performed. Results are expressed relative to the value of the NC group (set at 1 as the reference). (D) Representative western blot images for determining the expression of Bax, Bcl-2 and caspase 3, and the levels of p-PI3K and p-AKT protein. GAPDH was used as the loading control. (E) Quantitative analysis of relative changes in the proteins indicated above. **P<0.01. Groups: NC, cells transfected with NC siRNA; siRNA, cells transfected with H19 siRNA. NC, negative control; siRNA, small silencing RNA; p-PI3K, phosphorylated phosphoinositide-3 kinase; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2.