Figure 3.

Cardiac function is impaired in ischemic hearts, and Akt and p38 phosphorylation is decreased in primary cardiomyocytes after inhibition of miR-29a-3p. (A) Left ventricular fractional shortening and (B) relative wall thickness in CIR injury mice 5, 7 and 10 days after injections. (C) Western blotting and quantification of proteins in primary cardiomyocytes. Data were presented as mean ± standard error of the mean. n=5. *P<0.05 vs. respective controls. miR, microRNA; NC, negative control; Ad, adenovirus vector; Ad-miR-29a-3pi, adenovirus inhibiting miR-29a-3p; CIR, cardiac ischemia reperfusion.