In vitro proliferation of splenic lymphocytes from normal untreated mice and normal and immunosuppressed mice treated with BHP. (A) Proliferation rate. Proliferation was increased significantly in the MBHP and HBHP groups compared with that in the Con group, and increased significantly in the LBHP + Cy, MBHP + Cy and HBHP + Cy groups compared with that in the Cy group. (B) Stimulus index. The index was significantly increased in the HBHP group compared with that in the Con group, and significantly increased in the MBHP + Cy and HBHP + Cy groups compared with that in the Cy group. *P<0.01 vs. Con group; ***P<0.0001 vs. Con group; ###P<0.0001 vs. Cy group. All data were from two independent experiments, each of which was repeated in parallel twice. BHP, bioactive hepatic peptide; LBHP, low-dose BHP; MBHP, mid-dose BHP; HBHP, high-dose BHP; Cy, cyclophosphamide.