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. 2019 Jun 15;17:563–577. doi: 10.1016/j.omtn.2019.06.003

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Silencing of LINC00472 Facilitates the Proliferation, Migration, Invasion, and EMT of NCI-H1299 Cells

NCI-H1299 cells were transfected with sh-LINC00472 or sh-NC. (A) The transfection efficiency of the silencing of LINC00472 detected by qRT-PCR, normalized by GAPDH. (B) The expressions of EMT-related markers detected by western blot analysis, normalized by GAPDH. (C) Cell proliferation of each group detected by EdU assay (the original magnification is ×200). (D) Cell migration and invasion in each group detected by Transwell assay (the original magnification is ×200). *p < 0.05 versus the sh-NC group. The representative measurement data were expressed as mean ± SD. Comparisons between two groups were analyzed by unpaired t test. Comparisons among multiple groups were analyzed by one-way ANOVA. The experiment was repeated three times. LINC00472, long intergenic non-protein-coding RNA 472; NSCLC, non-small-cell lung cancer; EMT, epithelial-mesenchymal transition; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.