Figure 6.

Box5 blocked the morphine potentiation of gp120-induced pain. (a) Drug administration paradigm. In the simplified procedure, gp120 was i.t. injected on day 0 and morphine was i.p. administrated on days 0, 2, 4, and 6. Box 5 was i.t. delivered 30 min before gp120/morphine injection on days 0 and 6. (b) Hyperalgesia measured by von Frey tests. Identical to Figure 1(a), mechanical pain test was performed 5 h postmorphine injection. (c) Statistical comparison of the data points among groups in the shaded area in panel (b). After day 4, Box5 attenuated the potentiation effect of morphine on gp120-induced hyperalgesia (***p < 0.001; N.S., not significant; one-way analysis of variance with Tukey post hoc tests, n = 6).