Figure 7.

JQ1 and suberoylanilide hydroxamic acid (SAHA) synergistically inhibited tumor growth in vivo. A, NOZ cells (2 × 10⁶ in 200 μL PBS) were injected into the left axilla of each mouse. Three days later, the mice were randomly divided into 4 groups (negative control [NC], JQ1, SAHA and JQ1 + SAHHA) and treated with vehicle (10% DMSO and 90% of 10% 2‐hydroxypropyl‐β‐cyclodextrin, CAS 128446‐35‐5) alone, 50 mg/kg/d JQ1, 50 mg/kg/d SAHA or JQ1 + SAHA, respectively. All treatments were administered intraperitoneally 3 d per week (Monday, Wednesday and Friday) for 4 wk. B, Photo is presented to show the sizes of the tumors excised from the mice. C, The tumor volumes were measured by caliper and estimated (0.5 × width² × length) weekly. q values all > 1.0 indicate synergistic effects. D, Tumors were weighed. q value > 1.0 indicates synergy. E, Proteins were extracted from the tumors and BRD4, cyclin B1, cleaved caspase‐3, p‐AKT and p‐ERK1/2 expression levels were analyzed by western blot. All data are presented as mean ± SD. q value is shown as a column chart and: <1.0 indicates antagonism, =1.0 additivity, >1.0 synergy. Significant differences are indicated by *P < 0.05, **P < 0.01, and ***P < 0.001 vs negative control (NC); a: P < 0.05 JQ1 vs JQ1 + SAHA; b: P < 0.05, SAHA vs JQ1 + SAHA