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. 2019 Jul 26;10:1775. doi: 10.3389/fimmu.2019.01775

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Copyright © 2019 Muñoz González, Sycz, Alonso Paiva, Linke, Zorreguieta, Baldi and Ferrero.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anti-BtaF IgA antibodies in sera and mucosal samples. BALB/c mice were immunized with BtaF+c-di-AMP (BtaF) or saline+c-di-AMP (saline) at days 0, 7, and 14. One week after last immunization serum, saliva, feces, vaginal lavage, and bronchoalveolar (BAL) fluids and lung homogenates were obtained, and specific IgA antibody levels were measured by ELISA. Results are expressed as the mean OD_450nm + SD of three independent experiments. The asterisks indicate significant differences between the immunized group and the saline group. **p < 0.01.