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. 2019 Jul 18;18(3):3170–3176. doi: 10.3892/ol.2019.10636

Figure 4.

Figure 4.

miR-490-3p overexpression may degrade MAPK1. Dual luciferase reporter assay using (A) TE1 and (B) ECA109 cells demonstrated that miR-490-3p may bind to the 3′UTR of MAPK1. miR-490-3p overexpression significantly downregulated MAPK1 expression level in (C) TE1 and (D) ECA109 cells. miR-490-3p depletion significantly upregulated MAPK1 expression level. (E) miR-490-3p overexpression induced MAPK1 protein downregulation in ESCC cell lines. miR-490-3p depletion induced MAPK1 protein upregulation in ESCC cell lines. ***P<0.001. ESCC, esophageal squamous cell carcinoma; Luc, luciferase; MAPK1, mitogen-activated protein kinase 1; WT, wild-type; MT, mutant; miR, microRNA; NC, negative control.