Table 1. Comparison of incorporation mechanisms and efficiencies of uniformly labeled (UL) 13C-monosaccharides in intestinal Caco-2 and hepatic KKU-M213 cell lines.
13C-Saccharide | Incorporation mechanism |
% Incorporation |
% (Number of 13C/total number of C) |
|||||||
Man8GlcNAc2 |
Hex5HexNAc4Fuc1NeuAc1 |
Man8GlcNAc2 |
Hex5HexNAc4Fuc1NeuAc1 |
|||||||
Caco-2 | M213 | Caco-2 | M213 | Caco-2 | M213 | Caco-2 | M213 | Caco-2 | M213 | |
UL-13C-Glc | Glycolysis | Glycolysis | 86% | 98% | 86% | 97% | 91% | 97% | 86% | 95% |
UL-13C-Man | Glycolysis | Glycolysis & intact | 82% | 95% | 78% | 97% | 88% | 84% | 72% | 68% |
UL-13C-Gal | Glycolysis | Intact | 80% | 37% | 75% | 82% | 88% | 9% | 80% | 15% |
UL-13C-Fruc | Glycolysis | Glycolysis & intact | 84% | 91% | 76% | 92% | 86% | 86% | 72% | 83% |