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. 2019 Aug 1;19:760. doi: 10.1186/s12885-019-5963-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — CSC properties of sphere cells in fresh clinical HCC specimens and association between sphere formation and prognosis. a Representative photographs of spheres formed from 5 fresh clinical HCC specimens. Scale bar 50 μm. b Relative expression of CSC-related genes in 5 primary HCC spheres. The expression level of certain gene in sphere cells was normalized according to the expression of that in parental HCC cells. c Positive rates of sphere-formation in 25 patients stratified according to tumor size, number, satellite lesion, and tumor stage