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. 2019 Aug 1;19:55. doi: 10.1186/s12896-019-0549-z

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — End point LAMP molecular beacons at 75 degrees C. LAMP amplification at 64 degrees C for 30 cycles of 60 seconds, followed by 95 degrees C for 5 min and 75 degrees C for 5 min with fluorescence acquisition. Melt curve analysis between 60 degrees C and 95 degrees C. Template in blue, NTCs in orange. (1) 35Sp LAMP amplification with 35Sp LOOP MB, (2) 35Sp LAMP amplification with 35Sp STEM MB and (3) NOSt amplification with NOSt STEM MB. (A) real-time normalised fluorescence during amplification, (B) end point fluorescence at 75 degrees C, (C) melt profiles showing separation at 75 degrees C and (D) melt curve analysis with the melt temperature (Tm) of the molecular beacon