Table 5.
35S promoter STEM molecular beacons and SYTO9 detection of low copy number assay
| Detection Method | Amp. Freq. | Average Ct. | St. dev. | Fastest Ct. | Slowest Ct. |
|---|---|---|---|---|---|
| 4nt STEM MB | 25.80 | 31.06 | 12.74 | 21.91 | 83.33 |
| 5nt STEM MB | 26.80 | 32.90 | 12.74 | 24.10 | 80.06 |
| 6nt STEM MB | 32.40 | 35.26 | 10.65 | 25.02 | 65.16 |
| 7nt STEM MB | 18.30 | 46.29 | 13.17 | 34.29 | 75.74 |
| SYTO9 | 28.20 | 20.59 | 15.61 | 9.83 | 64.25 |
Diluted linear plasmid DNA containing the 35S promoter sequence assayed by 35Sp STEM molecular beacons of various lengths in comparison to the detection with SYTO9 fluorescence. LAMP assay at 64 degrees C with 1.0 micromolar LAMP molecular beacons and 72 replicates of 1 copy per partition. SYTO9 LAMP assay at 60 degrees C. The cycle parameters were set for 90 cycles of 60 seconds each cycle acquiring for FAM/SYTO9