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. 2019 Aug 1;21:86. doi: 10.1186/s13058-019-1166-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression of cytokeratin, CXCR4, and JUNB in CTCs isolated from breast cancer patients. a Quantification of CXCR4 expression (intensity per pixel) in breast cancer cell lines, Hela cells, PBMCs, and patients’ CTCs, using ARIOL system automate software. b Quantification of JUNB expression (intensity per pixel) in breast cancer cell lines, in Hela cells, PBMCs, and patients’ CTCs, using ARIOL system automate software. c Cytospins obtained from metastatic breast cancer patients were triple stained with CK (green), CXCR4 (orange), and JUNB (red) antibodies plus DAPI (blue) and analyzed with ARIOL system (magnification X40). d Representative images from confocal laser scanning microscopy. Patients’ samples were triple stained with CK (green), CXCR4 (red), and JUNB (blue) antibodies plus DAPI (not shown due to the absence of corresponding laser) magnification (× 60)