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. 2019 Jul 11;18(3):3104–3114. doi: 10.3892/ol.2019.10601

Table II.

tRFs and tiRNAs selected for validation via quantitative PCR.

Gene Primer sequence Product length, bp
U6 F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′ 89
R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′
AS-tDR-000064 F: 5′-CAGTCCGACGATCATCCCAC-3′ 45
R: 5′-TGCTCTTCCGATCTTGGTGG-3′
AS-tDR-000069 F: 5′-AGTTCTACAGTCCGACGATCTCTC-3′ 49
R: 5′-CTCTTCCGATCTTGGAGGTTC-3′
AS-tDR-000102 F: 5′-CTACAGTCCGACGATCTCCCC-3′ 45
R: 5′-TCTTCCGATCTTGGTGGAGATG-3′
AS-tDR-001391 F: 5′-GGCTCGTTGGTCTAGGGGTATG-3′ 52
R: 5′-ACGTGTGCTCTTCCGATCTGAA-3′

tRFs, transfer RNA-derived fragments; tiRNAs, tRNA halves.