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. 2019 Jul 9;18(3):2694–2703. doi: 10.3892/ol.2019.10589

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Rapa and Doxo induced cell apoptosis of K562 cells. Cells were incubated with Doxo (0.5 µM) and Rapa (20 nM) for 24 h. (A) Apoptosis of K562 cells was determined by Annexin V/PI apoptosis detection kit. Rapa and Doxo combination significantly increased apoptotic cells, compared with the matched single-drug group. (B) Western blotting showed the expressions of Bcl-2, Bax and Bcl-xL in Rapa, Doxo and the combination treatment group. The protein expression of (C) Bcl-2, (D) Bax and (E) Bcl-xL in K562 cells. Rapa and Doxo significantly decreased expression of (C) Bcl-2 and (E) Bcl-xL, and increase the expression of (D) Bax, respectively. *P<0.05. Data were presented as the mean ± standard deviation of triplicates. Rapa, rapamycin; Doxo, doxorubicin; FITC, fluorescein isothiocyanate; PI, propidium iodide.