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CA induced apoptosis in MDSCs in a dose-dependent manner. Adherent splenocytes were treated with different concentrations of CA (0, 1, 2 and 4 µg/ml) and DMSO for 48 h and then were stained with Annexin V-FITC and PI (20 µg/ml). (A) The stained cells were analyzed with a flow cytometer. (B) Bar chart demonstrating the percentages of apoptotic cells. ***P<0.001. CA, cinnamaldehyde; NS, not significant; OD, optical density.
