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. 2019 Jul 5;18(3):2491–2499. doi: 10.3892/ol.2019.10571

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Copyright: © Du et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — SiHa cell growth, cell cycle and invasion following miR-1246 downregulation. (A) miR-1246 expression in SiHa cells following infection with LV-miR-1246-Inh or LV-NC compared with untreated NC. (B) Proliferation of SiHa cells following miR-1246 downregulation. (C) Cell apoptosis detected by Annexin V-PE and 7-AAD labelling and flow cytometry. (D) Matrigel cell invasion assay showing the effects of LV-miR-1246-Inh on cell invasion. Magnification, ×200. (E and F) Cell cycle distribution determined by propidium iodide staining and flow cytometry highlighting the effects of LV-miR-1246-Inh on cell populations in the G1 and S phases. *P<0.05, **P<0.01, ***P<0.001. miR-1246, microRNA-1246; LV, lentivirus; Inh, inhibitor; NC, negative control; PE, phycoerythrin; 7-AAD, 7-aminoactinomycin D; OD, optical density.

Figure 1. — SiHa cell growth, cell cycle and invasion following miR-1246 downregulation. (A) miR-1246 expression in SiHa cells following infection with LV-miR-1246-Inh or LV-NC compared with untreated NC. (B) Proliferation of SiHa cells following miR-1246 downregulation. (C) Cell apoptosis detected by Annexin V-PE and 7-AAD labelling and flow cytometry. (D) Matrigel cell invasion assay showing the effects of LV-miR-1246-Inh on cell invasion. Magnification, ×200. (E and F) Cell cycle distribution determined by propidium iodide staining and flow cytometry highlighting the effects of LV-miR-1246-Inh on cell populations in the G1 and S phases. *P<0.05, **P<0.01, ***P<0.001. miR-1246, microRNA-1246; LV, lentivirus; Inh, inhibitor; NC, negative control; PE, phycoerythrin; 7-AAD, 7-aminoactinomycin D; OD, optical density.