Figure 5.

Matrine increases neurofilament-H (NF-H)-positive axons in injured spinal cord.

Sagittal sections of the spinal cords of vehicle-treated or matrine-treated were immunostained for NF-H. (A) Representative images at rostral and caudal sits are shown. Scale bar: 25 μm. (B) The diagram of analyzed area is shown. The measured areas in rostral and caudal sites were defined as the area 2.0–3.0 mm apart from the lesion center. (C, D) Ratio of NF-H-positive area to measured area was quantified at the rostral (C) and the caudal site (D). *P < 0.05, unpaired t-test (two-tailed). n = 5 mice (vehicle) or 6 mice (matrine).