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Construction of the murine TNF-α-producing transgenic C. neoformans strain. (A) expression construct containing C. neoformans ACTIN promoter and murine TNF-α cDNA sequences. (B,C) TNF-α expression by the transgenic strain H99-α was detected by RT-qPCR or by ELISA in the culture media at 37°C. (D) TNF-α level in the lung homogenates of mice infected with H99-α or H99 intratracheally at 48 h post-infection (Results represent mean ± SEM; *P < 0.05).
