Figure 2.

Expression of the murine TNF-α has no effect on cryptococcal fitness and major virulence factor production in vitro. (A) Growth curve of H99-α and H99 in liquid SDB media at 37°C. (B) Growth of H99-α and H99 on SD agar at 30°C (B) and 37°C (C), and agar plates containing H₂O₂ (D), NaCl (E), NaNO₂ (F), sorbitol (G). H99 and H99-α melanin production (H), urease activity (I), and capsule formation (J) with negative controls lac1Δ, ure1Δ, and cap59Δ for (H–J), respectively. Note that TNF-α expression by H99-α has not affected cryptococcal fitness or the expression of major virulence factor (Results represent mean ± SEM; ns, no Significance).