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. 2019 Jul 26;10:661. doi: 10.3389/fgene.2019.00661

Figure 7.

Figure 7

miR-206 targets the 3′-UTR of IGF-1. (A) Predicted binding site of miR-206 in the 3′-UTR of IGF-1. (B) Binding site of miR-206 is highly conserved among mammals. (C) IGF-1 3′-UTR was inserted into the pmirGLO dual-luciferase reporter vector at the 3′-end of the Renilla luciferase gene (hRluc). (D) IGF-1 3′-UTR or IGF-1 3′-UTR-Mutant construct was co-transfected with miR-206, miR-206_mut, or NC, as indicated, into PK cells, and normalized Renilla luciferase activity was determined. (E) Expression of miR-206 in the porcine skeletal muscle SCs infected with pri-miR-206 expression lentivirus or NC lentivirus. (F) Expression of IGF-1 mRNA in SCs and SCs infected with pri-miR-206 expression lentivirus or NC lentivirus. (G) Expression of IGF-1 protein in SCs and SCs infected with pri-miR-206 expression lentivirus or NC lentivirus. Results are mean ± SD (three independent replicates per group). *P < 0.05; **P < 0.01 (Student’s t test).