Fig. 3. (A) One-photon confocal fluorescence imaging of miR-21 in MCF-7, HeLa, and NIH-3T3 cells after incubation with ratioflares for 120 min. λex = 488 nm, λem(FAM) = 500–540 nm, λem(TAMRA) = 560–600 nm. Scale bar = 20 μm. (B) Average fluorescence intensity ratio between FAM channel and TAMRA channel after incubation for 120 min with ratioflares in three types of living cell. (C) FA/FD signal from cell-derived lysates of MCF-7, HeLa, and NIH-3T3 cells. Only telomerase-catalyzed FRET ratioflares were added to the control group.
