Fig. 4. One-photon confocal fluorescence imaging of miR-21 in HeLa cells after incubation with telomerase-catalyzed FRET ratioflares for 120 min. (A) group (a–c) was a control group, group (d–f) was treated with EGCG to inhibit the telomerase activity, group (g–i) did not contain catalyst strand. (B) group (j–l) was a control group, group (m–o) was treated with anti-miR-21 sequence to inhibit the miR-21 concentration, and group (p–r) was treated with contrastive probes. (C) Average fluorescence intensity ratio between FAM channel and TAMRA channel after incubating with EGCG and without CS. (D) Average fluorescence intensity ratio between FAM channel and TAMRA channel after incubating with anti-miR-21 sequence and contrastive probes. λex = 488 nm, λem(FAM) = 500–540 nm, λem(TAMRA) = 560–600 nm. Scale bar = 20 μm.
