Figure 1. Cytogenetics of Craterocephalus amniculus.

(A) Representative karyotype arranged from Giemsa-stained chromosomes. (B) C-banded chromosomes. The C-banding pattern was counterstained with DAPI and inverted; (C) Metaphase chromosomes after FISH with 5S rDNA (green signals) and 28S rDNA (red signals) probes; (D) CMA₃/DAPI stained chromosomes. The CMA₃ signal was converted from green into red and the DAPI signal from blue into green to enhance the contrast between these signals. (E) Metaphase chromosomes after FISH with a telomeric probe. Particular images were adjusted separately before assembling the plate. Arrows indicate the pair of homologous chromosomes with the accumulation of heterochromatin. Bars equal 10 µm.