TABLE 1.
Antibody Labeling
Antibody (dilution) | Immunogen | Source, host, catalog No., clone or lot No., RRID |
Validation | Staining pattern |
---|---|---|---|---|
Anticholine acetyltransferase (1:200) | Human placental enzyme | Chemicon/EMD-Millipore, goat polyclonal, AB144P, NG1915294, AB_2079751 | Western blot (immunoblot; Jordan et al., 2015) | Mammalian vestibular efferents (Kong et al., 1994; Jordan et al. 2015) |
Anticalretinin (1:200) | Recombinant rat calretinin | Chemicon/EMD-Millipore, rabbit polyclonal, B1550, 60302, AB_90764 | Western blot (immunoblot; Liu and Davis, 2014) | Mammalian type II vestibular hair cells and afferents (Desai et al., 2005; Jordan et al., 2015) |
Antiotoferlin (1:800) | Recombinant mouse peptide 1238-1391 (GenBank accession No. AAG12990) | Thermo Fisher Scientific, rabbit polyclonal, Custom_MorBar_001, AB_2616561 | Western blot (immunoblot; Ramakrishnan et al., 2014) | Mammalian cochlear and vestibular hair cells (Ramakrishnan et al., 2014); consistent with in situ hybridization pattern (Schug et al., 2006) |