Skip to main content
. 2019 Jul 24;10:1735. doi: 10.3389/fimmu.2019.01735

Figure 4.

Figure 4

The replication inhibition found in rt269I was mediated via STING-IFN-I axis. (A) IFNAR2 was blocked using anti-IFNAR2 antibody (10 μg) and HBV-encoding DNA and pSV-β-Galactosidase were transiently transfected into HepG2 cells. HBsAg and HBeAg in the presence of isotype or anti-IFNAR2 antibody were measured using ELISA at 48 h post-transfection. (B) HepG2 cells pre-treated with AZD1480 (5 μM) for 2 h were transfected with HBV DNA pSV-β-Galactosidase and constantly treated with AZD1480. HBsAg and HBeAg were measured using ELISA at 48 h post-transfection. (C) HBV DNA constructs and pSV-β-Galactosidase were co-transfected with either STING or scramble siRNA into HepG2 cells. The HBeAg levels and HBV virions were analyzed using ELISA and qPCR, respectively, at 48 h post-transfection. Data were normalized with β-Galactosidase enzyme assay, and represents mean ± S.D. of three independent experiments. One- and two-way ANOVA were used. *p < 0.05, **p < 0.01, ***p < 0.001.