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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: DNA Repair (Amst). 2019 May 3;79:32–39. doi: 10.1016/j.dnarep.2019.05.001

Fig. 4. Kinetics of temperature inactivation of WT and variants of NEIL1.

Fig. 4

WT and variants of NEIL1 A51V (A), P68H (B), and G245R (C) were pre-incubated at 37 °C and the activities were tested with equimolar DNA and enzyme concentrations (50 nmol/L) using TAMRA/BHQ2-conjugated double-stranded ThyGly substrate. The reactions were conducted at 37 °C and monitored by an Infinite M200 plate reader. The rates were calculated from the linear phase of reactions. The fraction of activity remaining at the given time point (A(t)) was calculated relative to the initial enzyme activity. The average A(t) values from at least three independent experiments were plotted as function of time (t). The uncertainties are standard deviations.