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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: DNA Repair (Amst). 2019 May 3;79:32–39. doi: 10.1016/j.dnarep.2019.05.001

Fig. 5. Removal of oxidatively-induced DNA base lesions by A51V following pre-incubation of enzyme at 37 °C.

Fig. 5

The γ-irradiated calf thymus DNA (50 μg) was incubated for 1 h at 37 °C in the presence of A51V enzyme (2 μg). The aliquot of A51V was pre-incubated at 37 °C for 30 min. Subsequently, DNA was added to the solution which was then incubated for another 30 min. The released FapyGua (A), FapyAde (B), and ThyGly (C) were measured by GC-MS/MS using their stable isotope-labeled analogues as internal standards (* = p < 0.05; ** = p < 0.01; *** = p < 0.001). The uncertainties are standard deviations.