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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Immunol Methods. 2019 Feb 6;467:37–47. doi: 10.1016/j.jim.2019.02.004

Figure 4: Peptain-1 is the preferred chaperone peptide for the antibody binding.

Figure 4:

Peptain-1 and the chaperone peptides of other small heat shock proteins were tested by a competitive ELISA (A) and Western blotting (B, left panel) as described in Methods. The gel run in parallel was stained with Coomassie blue (B, right panel). M=molecular weight markers, 1= peptain-1, 2=αA-crystallin, 3=Hsp20, and 4=Hsp27.