Fig 5. Conditioned media pathway analysis of the extracellular signaling molecules.

A) Pathway analysis of signaling molecules upregulated in the PH-CM. B) Comparison of the upregulated genes in the PH Cfib transcriptome (blue circle) with significantly enhanced proteins in the PH-CM (orange circle). Of the 137 secreted proteins found enriched in the conditioned media, the transcripts of 34 of these were also found increased in the transcriptome of the PH fibroblasts. C) Representative list of 16 of the 34 proteins identified as enriched in the PH conditioned media with known signaling properties that were also found upregulated in the PH Cfib transcriptome. The human orthologues are displayed in the table and were then used for pathway analysis.