Fig 7. Periostin drives adult myocyte dedifferentiation.

A) Periostin is significantly expressed in the PH-CM compared to CO-CM. B) Periostin RNA and C,D) protein expression are significantly higher in PH Cfib compared to CO. E) Immunodepletion of periostin from the PH-CM attenuates dedifferentiation, while addition of recombinant periostin to SF media stimulates dedifferentiation. F) Periostin expression is robustly upregulated in the dedifferentiated myocytes compared to freshly isolated ARVM. G) Periostin protein expression is upregulated in dedifferentiated cells compared to SF-treated. Periostin RNA and protein expression were assessed by qRT-PCR and immunoblotting, respectively. Experiments were performed in 3 PH and 2 CO calves in biological triplicate. *p<0.05 by student’s t-test.