Table 3. Titration of un-labelled competitor homologue quadrivalent DV NS1 quench.
| COHORT | G Capture | DABA | ||||||
|---|---|---|---|---|---|---|---|---|
| Unquenched | Quad Mix 25ug/ml S/CO |
Quad Mix 12.5ug/ml S/CO |
Quad Mix 6.25ug/ml S/CO |
Unquenched S/CO |
Quad Mix 25ug/ml S/CO |
Quad Mix 12.5ug/ml S/CO |
Quad Mix 6.25ug/ml S/CO |
|
| S/CO | ||||||||
| Dengue 2 | 2.184 | 0.384 | 0.493 | 0.623 | 12.278 | 0.513 | 0.536 | 0.632 |
| Dengue 2 | 8.421 | 0.439 | 0.6 | 0.778 | 6.57 | 0.528 | 0.572 | 0.641 |
| Dengue 2 | 4.379 | 0.544 | 0.754 | 1.472 | 11.858 | 0.491 | 0.557 | 0.632 |
| Dengue 2 | 6.926 | 0.485 | 0.571 | 0.759 | 9.968 | 0.543 | 0.68 | 0.784 |
| Dengue 2 | 8.574 | 0.453 | 0.799 | 0.858 | 6.091 | 0.468 | 0.557 | 0.637 |
| Dengue 2 | 3.011 | 0.475 | 0.526 | 0.594 | 4.608 | 0.445 | 0.546 | 0.582 |
| Dengue 2 | 1.426 | 0.485 | 0.654 | 0.67 | 5.301 | 0.687 | 0.722 | 0.559 |
| Dengue 2 | 4.337 | 0.686 | 0.857 | 1.075 | 0.77 | 0.468 | 0.5 | 0.486 |
| Dengue 2 | 2.279 | 0.457 | 0.447 | 0.467 | 4.421 | 0.347 | 0.433 | 0.426 |
| Dengue 2 | 6.405 | 0.517 | 0.547 | 0.665 | 0.977 | 0.589 | 0.577 | 0.591 |
| Dengue 2 | 4.268 | 0.37 | 0.576 | 0.585 | 8.252 | 0.558 | 0.588 | 1.072 |
| Dengue 2 | 2.642 | 1.573 | 1.723 | 2.024 | 2.214 | 0.408 | 0.536 | 0.431 |
| Dengue 2 | 5.453 | 0.562 | 0.675 | 0.783 | 1.864 | 0.543 | 0.619 | 0.646 |
| Dengue 2 | 6.611 | 0.539 | 0.671 | 0.807 | 9.221 | 0.445 | 0.531 | 0.486 |
| Dengue 3 | 2.021 | 0.59 | 0.621 | 0.693 | 1.95 | 1.245 | 0.938 | 0.948 |
| Dengue 3 | 9.416 | 0.777 | 0.915 | 0.91 | 14.375 | 0.725 | 0.768 | 0.756 |
| Dengue 3 | 3.337 | 0.709 | 0.634 | 0.679 | 2.229 | 0.702 | 0.711 | 0.591 |
| Dengue 4 | 2.226 | 1.207 | 1.379 | 1.429 | 12.66 | 0.506 | 0.588 | 0.49 |
| Dengue 4 | 2.553 | 0.736 | 0.762 | 0.717 | 25.864 | 0.528 | 0.608 | 0.564 |
| Dengue 4 | 9.947 | 0.594 | 0.683 | 0.788 | 1.663 | 0.521 | 0.572 | 0.577 |
| Mean S/CO | 4.821 | 0.629 | 0.744 | 0.869 | 7.157 | 0.563 | 0.607 | 0.627 |
| ZIKA | 3.505 | 3.237 | 2.882 | 3.09 | 5.794 | 5.894 | 4.196 | 3.216 |
| ZIKA | 6.221 | 4.649 | 4.617 | 4.915 | 6.212 | 2.702 | 2.088 | 1.865 |
| ZIKA | 12.621 | 5.888 | 6.621 | 7.887 | 8.607 | 2.694 | 2.113 | 1.746 |
| ZIKA | 12.937 | 7.991 | 8.062 | 9.123 | 5.713 | 4.325 | 1.387 | 2.465 |
Optimisation of the quadrivalent un-labelled competitor homologue DV NS1quench. Sample /cut off ratios are shown for both the G capture assay and the DABA. The sample reactivity unquenched and quenched at the three levels of 25, 12.5 and 6.25μg/ml is shown for each assay, samples still reactive in the presence of conjugate quenching are displayed in bold. Reactivity of the control ZIKA-positive samples is displayed (bottom four rows).