Skip to main content
. Author manuscript; available in PMC: 2019 Dec 24.
Published in final edited form as: Nat Med. 2019 Jun 24;25(7):1164–1174. doi: 10.1038/s41591-019-0461-z

Extended Data Fig. 7. Depletion of Treg cells abrogates protection by the microbiota.

Extended Data Fig. 7.

(a) Experimental schema. (b) Temperature changes in the respective OVA/SEB-sensitized and OVA-challenged mouse groups: Il4raF709Foxp3EGFP/DTR− (n=6), Il4raF709Foxp3EGFP/DTR−+Clostridiales+DT (n=7), Il4raF709Foxp3EGFP/DTR++Clostridiales+DT (n=8), Il4raF709Foxp3EGFP/DTR−+Bacteroidales+DT (n=9), Il4raF709Foxp3EGFP/DTR++Bacteroidales+DT (n=7). (c) Total and OVA-specific IgE in the groups listed in (b) (Total IgE: n=9, 6, 8, 7 and 5; OVA-specific IgE:6, 5, 5, 5, and 5 ). (d) MMCP-1 concentrations (n=12 for Il4raF709Foxp3EGFP/DTR−, and n=8 per group for all other groups). (e,f) Frequencies of MLN CD4+Foxp3+ and IL-4+Foxp3+ T cells [Il4raF709Foxp3EGFP/DTR− (n=6 and 10), Il4raF709Foxp3EGFP/DTR−+Clostridiales+DT (n=8 and 7), Il4raF709Foxp3EGFP/DTR++Clostridiales+DT (n=8 and 7), Il4raF709Foxp3EGFP/DTR−+Bacteroidales+DT (n=8 and 7), Il4raF709Foxp3EGFP/DTR++Bacteroidales+DT (n=8 and 7)]. (g) Frequencies of ROR-γt+Foxp3+ and GATA3+Foxp3+ T cells [Il4raF709Foxp3EGFP/DTR− (n=5 per group), Il4raF709Foxp3EGFP/DTR−+Clostridiales+DT (n=7 and 9), Il4raF709Foxp3EGFP/DTR++Clostridiales+DT (n=8 per group), Il4raF709Foxp3EGFP/DTR−+Bacteroidales+DT (n=6 per group), Il4raF709Foxp3EGFP/DTR++Bacteroidales+DT (n=8 pre group)]. Each dot represents one mouse. Data represent mean ± s.e.m. from two independent experiments. P values were derived by repeat measures two-way ANOVA (b), or by one-way ANOVA with Dunnett post hoc analysis or Student’s unpaired two tailed t test (c-f).